dna standard consisting of the microbial mock community b (even, low concentration)  (BEI Resources)

Journal: MicrobiologyOpen

Article Title: Differential association of key bacterial groups with diatoms and Phaeocystis spp . during spring blooms in the Southern Ocean

doi: 10.1002/mbo3.1428

Figure Lengend Snippet: The similarity of the bacterial communities in different size fractions collected from the four stations in three depth layers (20, 60–80, and 150 m) assessed by 16S rRNA marker gene analysis of DNA or RNA (cDNA) using Bray–Curtis distances and nMDS. The same nMDS is shown in two separate panels to show the separation of the samples by fraction and station (left) and by depth (right). Size fraction 0.8_micron (0.8–3 µm), 3_micron (>3 µm).

Article Snippet: We also included in parallel a control consisting of DNA from a synthetic mock community (Mock) of 20 bacterial species containing equimolar (even) rRNA operon counts (HM‐782D; Genomic DNA from Microbial Mock Community B, Even, Low Concentration, BEI Resources, Manassas, VA that was amplified as above with the bacterial primer pair.

Techniques: Marker

Journal: MicrobiologyOpen

Article Title: Differential association of key bacterial groups with diatoms and Phaeocystis spp . during spring blooms in the Southern Ocean

doi: 10.1002/mbo3.1428

Figure Lengend Snippet: Bacterial taxonomic diversity at the order level (DNA) at different depths for the four sampling stations in the 0.8–3 µm (0.8_micron) or >3 µm (3_micron) size fractions.

Article Snippet: We also included in parallel a control consisting of DNA from a synthetic mock community (Mock) of 20 bacterial species containing equimolar (even) rRNA operon counts (HM‐782D; Genomic DNA from Microbial Mock Community B, Even, Low Concentration, BEI Resources, Manassas, VA that was amplified as above with the bacterial primer pair.

Techniques: Sampling

Journal: MicrobiologyOpen

Article Title: Differential association of key bacterial groups with diatoms and Phaeocystis spp . during spring blooms in the Southern Ocean

doi: 10.1002/mbo3.1428

Figure Lengend Snippet: Alpha diversity of phytoplankton and bacterial communities in surface depths at the different stations converted into true diversities or “effective species number” (Jost, 2006 ) from the Shannon Index calculations (DNA).

Article Snippet: We also included in parallel a control consisting of DNA from a synthetic mock community (Mock) of 20 bacterial species containing equimolar (even) rRNA operon counts (HM‐782D; Genomic DNA from Microbial Mock Community B, Even, Low Concentration, BEI Resources, Manassas, VA that was amplified as above with the bacterial primer pair.

Techniques: Bacteria

Journal: MicrobiologyOpen

Article Title: Differential association of key bacterial groups with diatoms and Phaeocystis spp . during spring blooms in the Southern Ocean

doi: 10.1002/mbo3.1428

Figure Lengend Snippet: Impact of different factors on bacterial community structure tested by PERMANOVA analysis.

Article Snippet: We also included in parallel a control consisting of DNA from a synthetic mock community (Mock) of 20 bacterial species containing equimolar (even) rRNA operon counts (HM‐782D; Genomic DNA from Microbial Mock Community B, Even, Low Concentration, BEI Resources, Manassas, VA that was amplified as above with the bacterial primer pair.

Techniques:

Journal: MicrobiologyOpen

Article Title: Differential association of key bacterial groups with diatoms and Phaeocystis spp . during spring blooms in the Southern Ocean

doi: 10.1002/mbo3.1428

Figure Lengend Snippet: Bacterial community structure comparisons at depths 20 (1), 60–80 (2), 150 (3), and 300 m (4) at stations A3, F, E5, and R, recovered from two size fractions 0.8–3 µm (0.8) and >3 µm (3) assessed by 16S rRNA gene sequencing from DNA and RNA (RNA). Dissimilarity dendrograms were constructed using Bray–Curtis distances. The sample notation is StationName.SizeFraction.Depth for DNA samples and Name.SizeFraction. Depth.RNA for RNA samples.

Article Snippet: We also included in parallel a control consisting of DNA from a synthetic mock community (Mock) of 20 bacterial species containing equimolar (even) rRNA operon counts (HM‐782D; Genomic DNA from Microbial Mock Community B, Even, Low Concentration, BEI Resources, Manassas, VA that was amplified as above with the bacterial primer pair.

Techniques: Sequencing, Construct